Explain the step involved in paper chromatography .

Explanation

Separation of Chloroplast pigements by paper Chromatography method :
Step 1. Extract chlorophyll pigments from leaves using 80% Acetone .
Step 2. Allow to cconcentrate by evaporation .
Step 3. Apply few drops on one above 2 cm from the edge of a chromatographic paper .
Step 4 . A solvent with mixture of Petroleum ether and acetone in the ratio of 9:1 is prepared and poured into development chamber .
Step 5 . Place the strip above the solvent by placing one end of the Strip toching the solvent .
Observation : After one hour observe the chromatographic paper . You can find the pigments being separated into four distinct spots .