Explain Kuhne's fermentation experiment.

Explanation

Demonstration of alcoholic fermentation
Take a Kuhne's fermentation tube which consists of an upright glass tuve with side bulb. Pour 10% sugar solution mixed with baker's yeast into the fermentation tube the side tube is filled plug the mouth with lid. After some time, the glucose solution will be fermented .The solution will give out an alcoholic smell and level of solution in glass column will fall due to the accumulation of `CO_2` gas. It is due to teh presence of zymase enzyme in yeast which converts the glucose solution into alcohol and `CO_2`. Now introduce a pellet of KOH into the tube, the KOH will absorb `CO_2` and the level of solution will rise in upright tube.