Give the protocol for Micropropagation in Banana.

Explanation

Micropropagation protocol for banana
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In vitro micropropagation of banana (Musta sp. )
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Suckers surface sterilized with 1%
NaOCl for 30 minutes
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Apical meristems were isolated - cultured on
MS basal medium supplemented with
Benzylaminopurine (BAP) 10.0 mg/1 and
Indole Acetic (IAA) 1.0 mg/l
Induction of shoots within 168 days
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Kinetin 2.0 mg/l and Naphthalene Acetic Acid
(NAA) 0.5 mg/l for root induction
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Acclimatization in polyhouse
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Followed by shade-house under
50% light for hardening process
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tested for genetic uniformity
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Transferred to field
OR
Expand NBT and Explain how it is involved in developing new traits in plant breeding .
New. Breeding Techniques (NBT ) are a collection of methods that could increase and accelerate the development of new traits in plant breeding. these techniques often involve genome editing . to modigy DNa at specific locations within the plants to produce new traits in crop plants. the various methods of achieving these changes in traits include the following.
(i) Cutting and modifying the genome during the repair process by tools like CRISPR/Cas.
(ii) Genome editing to introduce changes in few base pairs using a technique called Oligonucleotide-directed mutagenesis (ODM).
(iv) Organising processes that alter gene activity without altering the DNA itself (epigenetic methods ).