Write a note on Replica plating technique of colony selection.

Explanation

(i) Replica plating technique is a method in which the pattern of colonies growing on a culture plate is copied.
(ii) A sterile filter plate is pressed against the culture plate and then lifted.
(iii) Then the filter plate is pressed against a second sterile culture plate
(iv) This results in the new plate being infected with cell in the same relative positions as the colonies in the original plate.
(v) The medium used in the second plate will differ from that used in the first.
(vi) The method may include an antibiotic or without a growth factor. Thus, transformed cells can be selected.