Explain Southern blotting techniques .

Explanation

The transfer of denatured DNA from Agarose gel to Nitrocellulose Blotting or Filter Paper technique was introduced by Southern in 1975 and this technique is called Southern Blotting Technique.
Steps
The transfer of DNA from agarose gel to nitrocellulose filter paper is achieved by Capillary Action. A buffer sodium saline Citrate (SSC) is used, in which DNA is highly soluble, it can be drawn up through the gel into the Nitrocellulose membrane.

By this process ss - DNA becomes . Trapped . in the membrane matrix.
This DNA is hybridized with a nucleic acid and can be detected by autoradiography.
Autoradiograph - A technique that captures the image formed in a photographic emulsion due to emission of light or radioactivity from a lalled component placed together unexposed