How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

Explanation

The microbial growth in culture medium can be overcome by autoclaving the medium at `121^(@)C` (15 psi) for 15 to 30 minutes.
Chemical sterilization using chemicals, sterilizaing using UV radiation. Alcoholic sterilization using ethanol, autoclaving and filtration etc., are the various techniques used to remove microbes.