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What are the optimal conditions that fav...

What are the optimal conditions that favours the induction of callus from nutrient medium?

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After the inoculation of explant in the nutrient medium supplemented with auxins and incubated at `25^(@)Cpm2^(@)C` in an alternate light and dark period of 12 hours with light intensity of 1000 lux, induces cell division leading to the development of callus from the surface of explant.
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