Explain how recombinant Insulin can be produced.

Explanation

Production of insulin by recombinantDNA technology started in the late 1970s. This technique involved the insertion ofhuman insulin gene on the plasmids of E.coli. The polypeptide chains are synthesized as a preeursor called pre-pro insulin, which contains A and B segments linked by a third chain (C) and preceded by a leader sequence. The leader scquence is removed arer translation and the C chain is excised, leaving the A and B polypeptide chains