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Elucidate the methodology of ELISA test....

Elucidate the methodology of ELISA test.

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During diagnosis the sample suspected to contain the antigen is immobilized on the surface of an ELISA plate. The antibody specific to this antigen is added and allowed to react with the immobilized antigen. The anti-antibody is linked to an appropriate enzyme like peroxidase. The unreacted anti-antibody is washed away and the substrate of the enzyme (hydrogen peroxidase) is added with certain reagents such as 4-chloronaphthol. The activity of the enzyme yields a coloured product indicating the presence of the antigen.
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