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What were the important discoveries that...

What were the important discoveries that led to the stepping stones of recombinant DNA technology?

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(a) Presence of plasmid in bacteria that can undergo replication independently along with chromosomal DNA
(b) Restriction enzymes cuts or break DNA at specific sites and are also called as molecular Scissors
(c) DNA ligases are the enzymes which help in ligating (joining) the broken DNA fragments
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