List three differences between Gram posotive and Gram negative bacteria.

Explanation

### Step-by-Step Text Solution

**Step 1: Identify the Structure of Cell Walls**
- **Gram Positive Bacteria**: They have a thick peptidoglycan layer in their cell wall, which is about 20 to 80 nanometers thick. They lack an outer membrane.
- **Gram Negative Bacteria**: They have a thinner peptidoglycan layer, approximately 8 to 10 nanometers thick, and possess an outer membrane.

**Step 2: Staining Characteristics**
- **Gram Positive Bacteria**: During the Gram staining process, they retain the crystal violet dye even after being washed with alcohol, resulting in a purple color when observed under a microscope.
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