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The enzyme that can join DNA fragments a...

The enzyme that can join DNA fragments are:

A

Exopeptidases

B

Ligases

C

Endonucleases

D

Topoisomerases

Text Solution

AI Generated Solution

The correct Answer is:
To answer the question, "The enzyme that can join DNA fragments are:", we can break down the solution into the following steps: ### Step 1: Understand the Options First, we need to analyze the provided options to identify which enzyme is responsible for joining DNA fragments. ### Step 2: Evaluate Each Option 1. **Exopeptidase**: This enzyme is involved in the digestion of proteins in the gastrointestinal tract. It cleaves di- and tri-peptides into amino acids. Therefore, it does not play a role in joining DNA fragments. 2. **Ligases**: Ligases are enzymes specifically responsible for joining DNA fragments. They catalyze the formation of phosphodiester bonds between the 3' hydroxyl group of one DNA fragment and the 5' phosphate group of another. This is crucial in recombinant DNA technology, where foreign DNA is inserted into a host DNA strand. 3. **Endonuclease**: While endonucleases are important for cleaving DNA at specific sites, they do not join DNA fragments. Instead, they create breaks in the DNA strands. 4. **Topoisomerase**: This enzyme helps in unwinding DNA by cutting and rejoining the DNA strands to relieve torsional strain. It does not join DNA fragments. ### Step 3: Conclusion Based on the evaluation of the options, the enzyme that can join DNA fragments is **Ligases**. ### Final Answer The enzyme that can join DNA fragments is **Ligases**. ---
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