Discuss in detail how RNA can be used to silence specific genes?
Hint: It can be done by process of RNA interference (RNA). It deals with cellular defense in eukaryotes. Here ds RNA binds to specific site in mRNA and silences it by checking its translation process.

A : RNAi takes place in all eukaryotic organisms as a method of cellular defense. R : Complementary dsRNA molecule binds to specific mRNA and prevents its translation (silencing).

Infestation of tobacco roots by the nematode Meloidegyne icognita can be prevented by this process . (I) DNA interference (II) RNA interference (III) using biopesticides. (IV) using bioinsecticides .

How does silencing of specific mRNA in RNA interference prevent parasitic infection?

Silencing of mRNA/RNA interference has been used in development of plants resistant to

Assertion: The nematode parasite could not survive in a transgenic plant expressing specific interfering RNA Reason :The sense & anti -sense RNA in host are complemenatry & forms a ds RNA, thus silenced the specific m RNA of nematod

Mark these statement as true (T) OR false (F) and select the correct option In eukaryotes,only one type of DNA-dependent RNA polymerase transcribes all types of RNAs The human gemone has approximately 30000 genes The untranslate regions (UTRs) of m-RNA are required for efficient translation process.

Which of the following statements are correct regarding the process of RNA interference ? (i) RNAi has been used to prevent nematode infestation of tobacco plants . (ii) RNAi takes place in all eukaryotic organisms as a method of cellular defense. (iii) The method involves silencing of specific mRNA using a complementary dsDNA molecule that binds and prevents the translation of mRNA. (iv) using retrovirus vectors , nematode–specific genes were introduced into the host plant .

Assertion: RNAi involves silencing of a specific mRNA due to complementary RNA that bind to & prevents translation of the mRNA Reason :RNAi takes place in all prokaryotic organisms as a method of cellular defense