`A_(3)-_(5)B`
`C_(5)-_(3)D`
AB and CD represent two strands of a DNA molecule.
When this molecule undergoes replication, a replication fork is formed between A and C in the above.
i. Name the template strands for replication.
ii. Using which strand as the template, will there be continuous synthesis of a complementary DNA strand?
iii. Complementary to which strand will Okazaki segments get synthesised.
iv. What are template strands and Okazaki pieces?
v. In which direction is a new strand synthesised?

Explanation

i. Complementary strands.
ii. One of the template strands with 3.`rarr`5. polarity. The synthesis of DNA is continuous.
iii. Other template strand with polarity 5.`rarr`3., the synthesis of DNA is discontinuous. i.e., short stretches of DNA are synthesised.
iv. Template - It is for the synthesis of a new complementary strand. Okazaki pieces: It is discontinuous fragments of DNA. It is Y shaped fragment.
v. The new strand is synthesised in 5.`rarr`3. direction.