[" In rDNA technology,antibiotics "],[" are used "],[" (A) As selectable markers "],[" (B) To detect alien DNA "],[" (C) To impart disease "],[" resistance to the host "],[" plant "],[" (D) To keep medium "],[" bacteria-free "]

Tools OF rDNA Technology, Vectors:- a)Ori b)Selectable Marker c)Cloning Sites d) Examples And Types OF Vector e)PBR322 Vector

Tools OF rDNA Technology|| Vectors:- a)Ori b)Selectable Marker c)Cloning Sites || Examples And Types OF Vector || PBR322 Vector

What could be the possible harm of using antibiotic resistant gene as a selectable marker in plasmid, used for gene transfer in production of GM food? (A) GM food contains the enzyme produced by the antibiotic resistance gene that was used during gene tranfer in genetic engineering. This could cause allergies since it is a foreign protein. (B) The bacteria present in the alimentary canal of the humans could take up the antibiotic resistance gene present in GM food. (C) The bacteria in the human alimentary canal would then become resistant to the concerned antibiotic. (D) The transgene may be transferred through pollen to their wild relattves and make weeds more persistant and damaging.

Identify the desirable characteristics for a plasmid used in rDNA technology from the following (a) Ability to multiply and express outside the host in a bioreactor (b) A highly active promoter (c) A site at which replication can be initiated (d) One or more identifiable marker genes (e) One or more unique restriction sites

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Following enzymes /techniques are used in the process of recombinant DNA technology A. Eco RI to cut the isolated genome B. DNA ligase C. Protease and ribonuclease for removal of proteins and RNA from DNA D. Production of recombination hosts E. lysozyme for isolation of the genetic material (DNA) F. gel electrophoresis for separation and isolation of DNA fragments Mark the correct sequence of their use.

Loss of biodiversity in a region can result in (A) Increased variability in plant productivity, water use, pests and disease cycle. (B) Decline in plant production (C) Lowered resistance to environmental perturbations How many of the above statements are correct

In genetic engineering, a DNA segment (gene) of interest, is translated to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors Statement (A) A bacterium (B) Plasmid (C) Plasmodium (D) Bacteriophage