[" Bacterial colonies that have a foreign "],[" DNA fragment inserted in to the "],[" plasmid will appear white because- "],[[" X-gal can be cleaved by "beta" - "],[" galactosidase "]],[" Lac-Z gene shows insertional "],[" inactivation due to insertion of "],[" foreign DNA "],[" Lac-Z gene is active "],[" X-gal can be cleaved by permease "]

An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA, helps in the selection of

An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA helps in the selection of

" In E-coli,if the gene for "beta" galactosidase enzyme is inactivated by insertion of DNA,then in presence of chromogenic substract recombinant will give "

Assertion: Recombinant colones will produce blue colour in the medium having X-gal compound Reason :The 'gal gene' of vecotr undergone insertional inactivation

X-gal is added in a culture medium containing recombi- nant plasmid, which has inactivated sequence for alpha -ga- lactosidase enzyme due to insertion of a foreign DNA. Which of the following condition will occur in this case?

Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour.

When we ligate a foreign DNA at the sa/l site of pBR322, the …....a…....plasmid will lose tetracycline resistance due to the insertion of foreign DNA but can still be selected out from …..b….. ones by plating the …...c…..... on ampicillin containing medium.