" In E-coli,if the gene for "beta" galactosidase enzyme is inactivated by insertion of DNA,then in presence of chromogenic substract recombinant will give "

In presence of chromogenic substrate recombinant bacteria gives

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

In insectional inactivation of beta -galactosidase gene, the bacteria in white colonies have

An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA, helps in the selection of

An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA helps in the selection of

If desired gene is inserted within the coding sequence of an enzyme, β-galactosidase in a vector then colonies of bacteria which do not produce any colour in the presence of a chromagenic substrates are :-

X-gal is added in a culture medium containing recombi- nant plasmid, which has inactivated sequence for alpha -ga- lactosidase enzyme due to insertion of a foreign DNA. Which of the following condition will occur in this case?

During gene cloning, the enzyme used to join and insert DNA within plasmid is

How are the structural genes inactivated in lac operon in E. Coli? Explain.

For selection of recombinants, insertional inactivation of antibiotic marker has been supercoded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.