DNA fragment with gene of interest and vector DNA are joined together by action of

DNA fragments are

When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky-ends and these can be joined together (end to end) using

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

In gentic engineering, DNA fragments are joined through

A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined.