Disease free plants are produced by

Options

A. Anther culture
B. Ovary culture
C. Shoot apex culture
D. Root apex culture

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks how disease-free plants are produced. This involves identifying the correct method or technique used in plant tissue culture that leads to the production of healthy, disease-free plants.

2. **Evaluating the Options**:
- **Anthraculture**: This method is primarily associated with hybridization and does not directly produce disease-free plants.
- **Ovary Culture**: Similar to anthraculture, this method is also used in hybridization and does not focus on producing disease-free plants.
- **Shoot Apex Culture**: This technique involves culturing the shoot or meristem tissue (the tip of the stem) in vitro. It allows for the introduction of disease-resistant genes, making it a viable method for producing disease-free plants.
- **Root Apex Culture**: While this method involves culturing the root, it does not have the same regenerative capabilities as shoot apex culture and is less effective in producing disease-free plants.

3. **Identifying the Correct Method**: After evaluating the options, it is clear that the most effective method for producing disease-free plants is **Shoot Apex Culture**. This technique utilizes the shoot meristem, which has high mitotic activity, allowing for the introduction of disease-resistant traits.

4. **Conclusion**: Therefore, the correct answer to the question is that disease-free plants are produced by **Shoot Apex Culture**.

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