In Ti-Plasmid, which of the following is removed?

Options

A. Auxin gene
B. Virulent gene
C. Cytokinin gene
D. Auxin and cytokinin gene

Answer

**Step-by-Step Solution:**

1. **Understanding Ti-Plasmid**: Ti-Plasmid stands for tumor-inducing plasmid, which is found in the bacterium Agrobacterium tumefaciens. This bacterium is known to cause crown gall disease in dicot plants by inducing tumor formation.

2. **Identifying the Components of Ti-Plasmid**: The Ti-Plasmid contains several important regions, including the T-DNA region, which is transferred to the host plant's cells, and the virulent region, which is responsible for tumor formation.

3. **Modification of Ti-Plasmid**: To use Ti-Plasmid as a cloning vector in genetic engineering, it must be modified. This involves removing the genes responsible for its pathogenic effects, specifically those that cause tumor formation.

4. **Which Genes are Removed?**: The virulent gene, which is responsible for the tumor-inducing properties of the Ti-Plasmid, is removed. This modification allows the plasmid to be used for transferring desired genes into the plant without causing disease.

5. **Conclusion**: Therefore, the correct answer to the question of which gene is removed from the Ti-Plasmid is the **virulent gene**.