Somatic hybridization can be done by:

Options

A. Protoplast fusion
B. Cell culture
C. Haploid anther
D. Polle culture

Answer

### Step-by-Step Solution

1. **Understanding Somatic Hybridization**:
- Somatic hybridization is a technique used to create hybrids from somatic (non-reproductive) cells of two different plants. This process allows for the combination of genetic material from different species or varieties.

2. **Isolation of Somatic Cells**:
- The first step in somatic hybridization is to isolate somatic cells from two different related varieties or species of plants. These cells can be taken from various parts of the plant, such as leaves or stems.

3. **Digestion of Cell Walls**:
- Once the somatic cells are isolated, the next step is to digest the cell walls. This is typically done using enzymes that break down the cellulose in the cell walls, resulting in the formation of protoplasts (cells without cell walls).

4. **Fusion of Protoplasts**:
- After obtaining protoplasts, the next step is to stimulate the fusion of these protoplasts. This can be achieved using various methods, such as electrical or chemical fusion techniques.

5. **Formation of Somatic Hybrid**:
- Once the protoplasts have fused, they form a hybrid cell known as a somatic hybrid. This hybrid cell contains genetic material from both parent plants.

6. **Example of Somatic Hybridization**:
- A well-known example of somatic hybridization is the creation of "Pomato," which is a hybrid obtained from the fusion of protoplasts from potato and tomato plants.

7. **Conclusion**:
- Somatic hybridization can be effectively carried out through protoplast fusion. Other methods mentioned, such as cell culture, haploid anther, and pollen culture, do not directly pertain to somatic hybridization.

### Final Answer
Somatic hybridization can be done by **protoplast fusion**.

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