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What modification is done on the Ti-plas...

What modification is done on the Ti-plasmid of Agrobacterium tumefacients to convert it into cloning vector?

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Fragment responsible for virulence has been removed. Tumour inducing gene has been kept intact as it is utilised for cloning and recognition site.
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What modification is done on the Ti-plasmid of Agrobacterium tumefaciens to convert it into cloning vector?

Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

How has Agrobacterium tumefaciens has been suitably modified to act as cloning vector?

Read the following statement and choose the correct option: I. Agrobacterium tumerfaciencs, a pathogen of several monocot plants, is able to deliver a piece of DNA known as "T-DNA". II. The tumor inducing (Ti) plasmid of Agrobacterium tumefaciens has been modified into a cloning vector, which is more pathogenic to the plants.

Which of the following statement // s regarding nucleic acids is // are wrong ? Amplification of gene of interest is done by polymerase Chain Reaction. Origin of replication (ori) is a sequence from where RNA replication starts. The tumour inducing (Ti) plasmid of Agrobacterium tumifaciens can be modified into a cloning vector . DNA technology involves the use of restriction endonucleases. Biolistics or gene gun is suitable to introduce DNA to plant cells.

What is the role of Ori for cloning vector?

Assertion (A) : Ti plasmid (tumour-inducing plasmid) of Agrobacterium tumefaciens is used as a cloning vector. Reason (R): Ti plasmid integrates a segment of its DNA, termed T-DNA into the chromosomal DNA of its host plant cells.

The Ti-plasmid of Agrobacterium tumefaciens is used to genetically engineering which of the following cell types ?

(A) : Agrobacterium Ti plasmid is used to induce genetic transformation in plants. (R) : Agrobacterium tumefacients usually causes crown gall disease in monocotyledons.