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A plasmid DNA and linear DNA (both are o...

A plasmid DNA and linear DNA (both are of the same size) have one site for restriction endonuclease. When cut and separated on agarose get electrophoresis, plasmid shows one DNA b and, while linear DNA shows two fragments Explain.

Text Solution

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From the Fig. given below, it is obvious when plasmid DNA is cut, it turns linear. However, it fails to form fragments. On the other hand linear DNA when treated with enzyme forms two fragments.
Thus, for plasmid, one DNA band appears and for linear DNA two DNA bands appear
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