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A recombinant DNA molecule can be produc...

A recombinant DNA molecule can be produced in absence of E.coli/Restriction endonuclease.

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To answer the question, "A recombinant DNA molecule can be produced in the absence of E. coli or Restriction endonuclease," we can follow these steps: ### Step-by-Step Solution: 1. **Understanding Recombinant DNA**: - Recombinant DNA (rDNA) is DNA that has been artificially created by combining DNA from different sources. This process alters the genetic makeup of the DNA. 2. **Role of Restriction Endonucleases**: ...
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A recombinant DNA molecule can be produced in the absence of the followingt :

In recombinant DNA technology before the use of restriction endonuclease , we have to isolated and precipitate the genetic material (DNA) . For this purpose we use following different enzyme / chemical :

Assertion: Recombinant DNA technology has become successful because of the presence of restriction endonucleases in eukaryotic cells. Reason: Restriction endonucleases cut the DNA molecule to form blund ends.

(a) Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA. (b) Write the use of restriction endonuclease in the process.

DNA of E.Coli :

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Which type of restriction endonucleases is used most in genetic engineering?

Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Restriction endonuclease was isolated for the first time from a: