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DOWNSTREAM PROCESSING

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Products formed are separated and purified. Steps are collectively called as .downstreaming processing.
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Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out fron agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

From given statements select the incorrect statement. (a) Proteins which are obtained by recombinant DNA technology are called recombinant proteins (b) Downstream processing include separation and purification of the desired product (c) After purification the product has to be formulated with suitable preservatives (d) Downstream processing and quality control testing do not vary from product to product

Read the following statements and select the incorrect ones. (i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells with grow. (ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules. (iii) Downstream processing is one of the steps of rDNA technology. (iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host.

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Read the following four statements (A-D) A. The first transgenic buffalo, Roise produced milk which was human alpha-lactalbumin enriched B. Restriction enzymes are used in isolation of DNA from other macromolecules C. Downstream processing is one of the step of rDNA technology D. Disarmed pathogen vectors are also used in transfer of rDNA into the host which of the two statements have mistakes ?

Read statements a-d. Which two of them have mistakes (a) First transgenic buffalo Roise produced milk which was human alpha-lactalbumin enriched (b) Restriction enzymes are used in isolation of DNA from other macromolecules (c) Downstream processing is one of the steps of rDNA technology (d) Disarmed pathogen vectors are also used in transfer of rDNA into the host

biotechnology principles and processes || biotechnology and its applications || restriction endonucleases || DNA ligases || vector || competent host || bioreactors || downstreaming || application in medicine and agriculture || bioethics || biopatency || biopiracy || bioweapons