How is insertion of recombinant DNA done in host cell (organ)?

Which is not a method for the introduction of recombinant DNA into host cells?

Explain any two methods for introduction of recombinant DNA into host cell.

What is the process of insertion of recombinant DNA in bacterial cell ?

Amplification OF the Gene OF Interest using PCR|| Ligation OF the DNA fragment into a Vector|| Insertion OF Recombinant DNA into the Host cell/organism

Assertion : Special methods are used for transformation i.e., incorporation of recombinant DNA into host. Reason : DNA is a hydrophilic molecule.

Which of the following can be used to insert the recombinant DNA into the animal cell?

DNA being hydrophilic cannot pass through the cell membranes of a hot cell. Explain how does recombinant DNA get introduced into the host cell to transfrom the latter.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introducting of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Which of the following process is used to insert recombinant DNA directly into the nucleus of the animal cell ?