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Rearrange the following in the correct s...

Rearrange the following in the correct sequence to accomplish an important biotechnological reactions :
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(C) Enzyme DNA-polymerase
(d) complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymers (from Thermus aquaticus)
(i) Denaturation of ds-DNA

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Rearrrange the following in the correct sequence to accomplish an important biotechnological reaction: ( a) In vitro synthesis of copies of DNA of interest (b) Chemically synthesized oligo - nucleqtides ( c) Enzyme DNA - polymerase ( d) Complementary region of DNA ( e) Gernomic DNA template (f) Nuceltides provided ( g)Primes (h) Thermostable DNA -polymerase (from Thermus aquaticus ) ( i) Denaturation of ds- DNA

Rearrange the following in the correct sequences to accomplish an important biotechnological reaction: a) In vitro synthesis of copies of DNA of interest b) Chemically synthesized oligonucleotides. c) Enzymes DNA polymerase c) Complementary region of DNA. e) Genomic DNA template f) Nucleotides provided. g) Primers h) Thermostable DNA-polymerase (from Thermus aquaticus). i) Denaturatioin of ds-DNA.

Among the following, select the tools of recombinant DNA technology a. Restriction enzymes b. Polymerase enzymes c. Ligases d. Vectors e. Host organisms

The synthesis of RNA primer complimentary to the DNA template strand is assisted by: a) Primase b) DNA polymerase III c) Helicase d) Ligase

Which of the following statement are correct for the enzyme Taq polymerase? (i) It remains active during the high temperature . (ii) It requies primers for carrying out the process of polymerization. (iii) It induces denaturation of double-stranded DNA.

Which of the following is/are part of biotechnology? A. Correcting a defective gene . B.Developing a DNA vaccine C. In vitro fertilisation D.Synthesis of a gene.

Consider the following statements (A). r-RNA provides the template for synthesis of proteins (B) tRNA brings amino acids and reads the genetic code (c ) RNA polymerase binds to promother and initiates transcription (D) A segment of DNA coding for polypeptide is called intron

A schematic representation of polymerase chain reaction (PCR) up to the extension stage is given below. Which of the following has popularized the PCR (Polymerase Chain Reaction) ? a) Easy availability of DNA template b) Availability of synthetic primers c) Availability of cheap deoxyribonucleotides d) Availability of thermostable DNA polymerase.

Which of these statements about protein synthesis are NOT correct ? I. During transcription, mRNA synthesized from DNA nucleotides to have the same sequence of nucleotides as the DNA strand on which it was made. II. During transcription , tRNA is synthesized from RNA nucleotides and carries codons that are complementary to the sequence of nucleotides on the DNA strand on which it was made. III. During translation, mRNA is synthesized from RNA nucleotides to have the complementary sequence of nucleotides to that. Of the DNA strand on which it was made . IV. During translation ribosomes move on mRNA from '5' and '3' one codon at a time .This is called translocation

MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -REVISION EXERCISES (IV. SHORT ANSWER TYPE -II QUESTIONS ( A. QUESTION FROM STATE BOARD EXAMINATIONS :))
  1. Discuss direct method of gene transfer.

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  2. How can bacterial cell be made competent to take up DNA?

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  3. Rearrange the following in the correct sequence to accomplish an impor...

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  4. Explain briefly polymerase chain reaction.

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  5. Define Genetic Engineering. Mention the steps of Genetic Engineering P...

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  6. Explain the role(s) of the following Biotechnology: a) Restriction e...

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  7. Write the stpes you would suggest to be undertaken to obtain a foreign...

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  8. Write a brief account of genetically engineered Insulin

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  9. What is EcoRI? Write the palindromic nucleotide sequence recognized by...

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  10. Why is it difficult for DNA to pass through cell membrane? How is a ba...

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  11. How are the desired DNA sequence used in biotechnology cut? Explain th...

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  12. Name the selectable markers in the cloning vector pBR322? Mention the ...

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  13. What is a cloning vector? Describe briefly the characteristics a cloni...

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  14. A small fragment of skin of a different person was extracted from the ...

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  15. What do you mean by GM organisms? Mention any three advantages of GM o...

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  16. What is recombinant DNA technology? What are its applications?

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  17. What are plamids? What is their role in biotechnology?

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  18. (a) Differentiate YAC and BAC. (b) What are Bioreactors? What is th...

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  19. What are molecular scissors? Give an example.

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  20. What are molecular scissors?

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