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What is the function of Restriction Endo...

What is the function of Restriction Endonuclease in recombinant DNA technology?

A

Link together fragments of DNA

B

Make millions of copies of DNA

C

Cut DNA into many fragments

D

Separate fragments of DNA

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Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called as molecular scissors or biological scissors. Three types of restriction endemicleases are Type 1. Type II and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A. Which type of restriction endonucleases is used most in genetic engineering?

MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -REVISION EXERCISES (IV. SHORT ANSWER TYPE -II QUESTIONS ( A. QUESTION FROM STATE BOARD EXAMINATIONS :))
  1. Write the stpes you would suggest to be undertaken to obtain a foreign...

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  2. Write a brief account of genetically engineered Insulin

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  3. What is EcoRI? Write the palindromic nucleotide sequence recognized by...

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  4. Why is it difficult for DNA to pass through cell membrane? How is a ba...

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  5. How are the desired DNA sequence used in biotechnology cut? Explain th...

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  6. Name the selectable markers in the cloning vector pBR322? Mention the ...

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  7. What is a cloning vector? Describe briefly the characteristics a cloni...

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  8. A small fragment of skin of a different person was extracted from the ...

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  9. What do you mean by GM organisms? Mention any three advantages of GM o...

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  10. What is recombinant DNA technology? What are its applications?

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  11. What are plamids? What is their role in biotechnology?

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  12. (a) Differentiate YAC and BAC. (b) What are Bioreactors? What is th...

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  13. What are molecular scissors? Give an example.

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  14. What are molecular scissors?

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  15. Describe the essential steps in Genetic Engineering.

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  16. What is the function of Restriction Endonuclease in recombinant DNA te...

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  17. (a) Explain PCR. (b) How desired DNA is isolated in recombinant DNA ...

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  18. PCR and ELISA are two molecular diagnostic techniques. (a) How is P...

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  19. Many countries encourage the cultivation of Genetically Modifed Crops ...

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  20. With reference to gel electrophoresis, what is elution?

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