(i) Name the organism in which the vector shown in inserted to get the copies of the desired gene.
(ii) Mention the area labelled in the vector responsbile for controlling the copy number of the inserted gene.
(iii) Name and explain the role of a selectable marker in the vector shown.

The techniques of insertion of a desired gene into DNA of plasmid vector is

The technique involving insertion of a desired gene into the DNA of plasmid vector is know as

Name the commonly used vector for cloning genes into higher organisms.

Name the selectable markers in the cloning vector pBR322? Mention the role of they play.

(a) Name the selectable markers in the cloning vector pBR322 ? Mention the role they play. (b) Why is the coding sequence of an enzyme b-galactosidase a preferred selectable marker in comparison to the ones named above ?

The given figure is the diagrammatic representaton of the E. coli vector pBR322. Which of these genes can act as selectable marker ?

If a desired gene is identified in an organism for some experiments, explain the process of the following (i) Cutting this desired gene at specific location (ii) Synthesis of multiple copies of this desired gene

Mention the role of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introducting of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?