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Discuss the role, the enzyme DNA ligase ...

Discuss the role, the enzyme DNA ligase plays during DNA replication.

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Explain the mechanism of DNA repication with the help of a replication fork. What role does the enzyme DNA-ligase play in a DNA replication fork ? OR Construct and label a transcription unit from which the RNA segment given below has been transcribed. Write the complete name of the enzyme that transcribed this RNA.

During replication of DNA

Draw a labelled schematic sketch of replication fork of DNA. Explain the role of the enzymes involved in DNA replication.

Draw a labelled schematic sketch of replication fork of DNA. Explain the role of the enzymes involved in DNA replication

DNA synthesis during replication is

What are DNA ligases?

The replication process involves the following steps: (I) The enzyme DNA-dependent DNA poly merases catalyse the polymerisation of DNA strand. (II) The lagging strand is synthesized in frag ments known as Okazaki fragments, which are later joined by the enzyme DNA Ligase. (III) The origin of replication serves as the recognition site for DNA polymerase and also it provides the site for attachment of RNA primer. (IV)The structural gene coding for a partic ular protein is flanked by the promoter and terminator. Choose the correct option:

(a) Name the stage in the cell cycle where DNA replication occurs. (b) Explain the mechanism of DNA replication. Highlight the role of enzymes in the process. (c) Why is DNA replication said to be semiconservative ?

MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -REVISION EXERCISES (IV. SHORT ANSWER TYPE -II QUESTIONS ( B. QUESTION FROM CBSE BOARD EXAMINATIONS :))
  1. (a) What does this diagram depict? (b) What is meant by the largest...

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  2. (i) Name the organism in which the vector shown in inserted to get the...

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  3. Draw a schematic sketch of pBR 322 plasmid and label the following in ...

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  4. A vector is engineered with three features which facilitate its clonin...

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  5. Discuss the role, the enzyme DNA ligase plays during DNA replication.

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  6. Explain enzyme-replacement therapy to treat adenosine deaminase defici...

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  7. (a) Name the selectable markers in the cloning vector pBR322 ? Mention...

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  8. Describe the roles of heat , primers and the bacterium Thermus aquatic...

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  9. Explain the various steps involved in the productin of artifical insul...

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  10. (a) Explain the significance of palindromic nucleotide sequence in th...

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  11. a) While cloning vectors, which of the two will be preferred by biotec...

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  12. a) How do DNA fragments migrate and resolve in a Gel electrophoresis? ...

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  13. (a) Why must a cell made 'compelent' in biotechnology experiments ? Ho...

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  14. What is Ori? State its importance during cloning of vector.

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  15. Explain the importance of 'selectable marker' with the help of suitabl...

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  16. Given below is the diagram of agarose gel kept under UV light: (...

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  17. (a) In pBR322, foreign DNA has to be introduced in Tet^(R) region. Fro...

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