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Describe the roles of heat , primers and...

Describe the roles of heat , primers and the bacterium Thermus aquaticus in the process of PCR.

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MODERN PUBLICATION-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES -REVISION EXERCISES (IV. SHORT ANSWER TYPE -II QUESTIONS ( B. QUESTION FROM CBSE BOARD EXAMINATIONS :))
  1. (a) What does this diagram depict? (b) What is meant by the largest...

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  2. (i) Name the organism in which the vector shown in inserted to get the...

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  3. Draw a schematic sketch of pBR 322 plasmid and label the following in ...

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  4. A vector is engineered with three features which facilitate its clonin...

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  5. Discuss the role, the enzyme DNA ligase plays during DNA replication.

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  6. Explain enzyme-replacement therapy to treat adenosine deaminase defici...

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  7. (a) Name the selectable markers in the cloning vector pBR322 ? Mention...

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  8. Describe the roles of heat , primers and the bacterium Thermus aquatic...

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  9. Explain the various steps involved in the productin of artifical insul...

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  10. (a) Explain the significance of palindromic nucleotide sequence in th...

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  11. a) While cloning vectors, which of the two will be preferred by biotec...

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  12. a) How do DNA fragments migrate and resolve in a Gel electrophoresis? ...

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  13. (a) Why must a cell made 'compelent' in biotechnology experiments ? Ho...

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  14. What is Ori? State its importance during cloning of vector.

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  15. Explain the importance of 'selectable marker' with the help of suitabl...

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  16. Given below is the diagram of agarose gel kept under UV light: (...

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  17. (a) In pBR322, foreign DNA has to be introduced in Tet^(R) region. Fro...

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