(a) Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
(b) Write the use of restriction endonuclease in the process.

Explain the process involved in the production of recombinant DNA .

A recombinant DNA molecule can be produced in absence of E.coli/Restriction endonuclease.

Write a specific palindromic nucleotide sequence in DNA that is recognised by Eco RI. (b) What does Eco RI stand for?

With the help of diagrams show the different steps in the formation of reeombinant DNA by action of restriction endonuclease enzyme EcoRI. Name the technique that 18 used for separating the fragments of DNA cut by restriction endonncleases.

Read the given statements and select the correct option, Statement 1 : Restriction endonuclease enzymes recongnise a specific palindromic nucleotide sequence in the DNA Statement 2: Restriction endonuclease enzymes are called as molecular scissors or biological scissors.

Assertion: Each restriction endonuclease recognises a specific pallindromic nucleotide sequence in the DNA Reason :The pallindrome in DNA is a sequence of base pairs that reads same on the two strands in a particular direction

Name and describe that technique that helps in separating the DNA fragments formed by the use of restriction endonuclease.

Describe the formation of recombinant DNA by the action of EcoRI. OR Describe the process of amplification of "gene of interest" usning PCR technique.

The number of nucleotides present in the restriction sequence of the first restriction endonuclease is