(a) In pBR322, foreign DNA has to be introduced in `Tet^(R)` region. From the restriction enzymes given below, which one should be used and why: Pvul, EcoRI, BamHI. (b) Give reasons, why the other two enzymes cannot be used.

Which one of the following restriction enzyme can cut "Tet"^R site of pBR322 ?

Assertion : Unless one cuts the vector and the foreign/source DNA with the same restriction enzymes, the recombinant vector molecule cannot be created Reason: When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together (end to end) using DNA ligases

A plasmid has two antibiotic-resistance genes, one for ampicillin and one for tetracycline. It is treated with a restriction enzyme that cuts in the middle of the ampicillin gene. DNA fragments containing a haemoglobin gene were cut with the same enzyme. The plasmids and fragments are mixed, treated with ligase and used to transform bacterial cells. Clones that have taken up the recombinant DNA are the one that

Assertion : Recognition site should be perfectly single and responsive to commonly used restriction enzymes. Reason: In pNR 322 Alien DNA is ligated generally in the area of Bam-HI site of tetracycline resistance gene.

Read the following and choose the correct statements . (A) First recombination DNA - Plasmid isolated from Salmonella and tagged with antibiotic resistance gene and cultured in E.coil. (B) Eco RI- It recognises a palindrome with 6 base pairs and 14 hydrogen bonds . (C) pBR 322 - 8 restriction sites for eigth types of restriction enzyme , two types of gene for antibiotic resistacne , one for gene replication of plasmid (Ori) . (D) Copy number - Ori gene that supports the production of more DNA copies .