Explain PCR. How it helps in amplification of gene of interest?

Gene amplification.

Describe the process of amplification of gene of interest

Amplification Of Gene Of Interest Using Pcr

Describe the formation of recombinant DNA by the action of EcoRI. OR Describe the process of amplification of "gene of interest" usning PCR technique.

How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)?

Explain the contributin of Thermus aquaticus in the amplification of a geme of interest.

The whole cycle of gene amplification requires

(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

Consider the following statements with respect to PCR. A. PCR is now routinely used to detect HIV in suspected AIDS patients. B. PCR is being used to detect mutations in genes in suspected cancer patients. C. PCR involves the amplification of mRNA. D. In PCR repeated amplification of RNA is achieved by the use of a thermostable RNA polymerase. Of the above statement: