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Explain the importance of a) ori, b) amp...

Explain the importance of a) ori, b) `amp^(R )` and c) rop in the E, coli vector shown below:

Text Solution

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(a) Ori: Origin of replication necessary for replication of plasmid.
(b) ampR: Ampicillin antibiotic resistance gene. It is required for separation of recombinant DNA from non-recombinant.
(c) rop: The gene which forms proteins involved in replication of plasmid.
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