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What is RNA interference (RNAi)? Explain...

What is RNA interference (RNAi)? Explain in correct sequence the use of this process in producing nematode resistant tobacco plants.

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Which of the following statements are correct regarding the process of RNA interference ? (i) RNAi has been used to prevent nematode infestation of tobacco plants . (ii) RNAi takes place in all eukaryotic organisms as a method of cellular defense. (iii) The method involves silencing of specific mRNA using a complementary dsDNA molecule that binds and prevents the translation of mRNA. (iv) using retrovirus vectors , nematode–specific genes were introduced into the host plant .

The process of RNA interference has been used in the development of plants resistant to

MODERN PUBLICATION-BIOTECHNOLOGY AND ITS APPLICATIONS-REVISION EXERCIES (LONG ANSWER TYPE QUESTIONS)
  1. (a) What is DNA fingerprinting? (b) Explain the steps involved in DN...

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  2. Write the salient features of Human Genome Project.

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  3. What do Genetically Modified Organisms (GMO) do to the food? How do th...

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  4. Give an account of the production of human insulin in transgenic org...

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  5. Write an account of biotechnological applications in medicine.

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  6. What is polymerase chain reaction (PCR) ? Explain in detail the steps ...

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  7. Describe the salient features of human genome.

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  8. What are Cry proteins? How has biotechnologist exploited these protein...

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  9. Briefly write about: (a) Downstream processing (b) Bioreactors.

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  10. With an example, explain how biotechnology has been applied in each of...

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  11. How is Bt cotton plant produced? Explain the mechanism by which the pl...

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  12. What is RNA interference (RNAi)? Explain in correct sequence the use o...

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  13. (a) Name the nematode that infests and damages tobacco roots. (b) Ho...

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  14. (a) Name the source of Taq polymerase. Explain the advantage of its us...

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  15. One of the main objectives of biotechnology is to minimize the use of ...

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  16. How is mature insulin different from proinsulin secreted humans pancr...

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  17. a) Explain DNA polymorphism as the basis of genetic mapping of human g...

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  18. (a) What do 'Y' and 'B' stand for in 'YAC' and 'BAC' used in Human Gen...

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  19. (a) Why should a bacterium be made 'competent? (b) Explain the role ...

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  20. Write any six salient feactures of the human genome as drawn from the ...

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