What are the major requirements be Chemiosmosis to occurs ?

Explanation

### Step-by-Step Solution:

1. **Understanding Chemiosmosis**: Chemiosmosis is a process that involves the movement of protons (H⁺ ions) across a membrane, which generates energy used to synthesize ATP. This process is crucial in cellular respiration and photosynthesis.

2. **Proton Pump**: The first major requirement for chemiosmosis is the presence of a proton pump. This pump actively transports protons from the mitochondrial matrix (or chloroplast stroma) into the intermembrane space (or thylakoid lumen), creating a proton gradient.

3. **Proton Gradient**: The second requirement is the establishment of a proton gradient. This gradient is created by the differential concentration of protons on either side of the membrane. The side with a higher concentration of protons has potential energy that can be harnessed.

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