Gemini opened the fridge in her home and found that a small bottle labelled with rDNA insulin
a. Is it a natural insulin?
b. Identify the major steps involved in this rDNA insulin production.

Explanation

a. No b. First two DNA sequences corresponding to A and B chains of human
insulin are prepared. Then they are introduced into plasmids of E.coli to pruduce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin