Due to difficulty in inactivation of antibiotics, alternate selectable markers developed to differentiate recombinants from non-recombinants on the basic of ability to

Alternate selectable markers have ability to differentiate recombinants from non-recombinants due to the

How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?

In 'Lac' selection method of alternative selectable marker technique which of the following marker used for identification of recombinants from non recombinations (A) amp^(R) gene (B) tet^(R) gene (C) Lac z^(1) gene (D) x-gal

Explain the importance of (a) ori, (b) amp^R and (c ) rop in the E. coli vector shown below: ampR:Ampicillin antibiotic resistance gene. It is required for separation of recombinant DNA from non-recombinant. rop: The gene which forms proteins involved in replication of plasmid.

If PBR322 is cleaved with Bam HI to insert foreign DNA,which of the following selectable marker is used "],[" to screen recombinants from non- "],[" recombinants?

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.