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The bacteria which do not retain the cry...

The bacteria which do not retain the crystal violet of iodine stain when after washing with alcohol are included in

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Assertion : Gram-negative bacteria do not retain the stain when washed with alcohol. Reason : The outer face of the outer membrane of Gram-negative bacteria contains lipopolysacharides, a part of which is integrated inot the membrane lipids.

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Assertion : Some bacteria have the caoacity to retain Gram stain after treatement with acid alcohol. Reason : They are known as Gram positive as they are attracted towards positive pole under influence of electric current.