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In presence of chromogenic substrate rec...

In presence of chromogenic substrate recombinant bacteria gives

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" In E-coli,if the gene for "beta" galactosidase enzyme is inactivated by insertion of DNA,then in presence of chromogenic substract recombinant will give "

In a bacterial culture, some of the colonies produced blue colour in the presence of a chromogenic substrate and some did not due to the presence or absence of an insert (rDNA) in the coding sequence of beta - galactosidase. (a) Mention the mechanism and the steps involved in this experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics?

In bacterial culture some of the colonies produced blue colour in the presence of chromogenic substrate and some did not due to presence or absence of an inert (rDNA) in the coding sequence of B galactosidase. (a) Mention the mechanism and steps involved in above experiment. (b) How is it advantageous over simultaneous plating on two plates having different antibiotics.

Select the wrong statement (a) Presence of chromogenic substrate gives blue color colonies if the plasmid in the bacteria does not have an insert (b) Retroviruses in animals have the ability to transform normal cells into cancerous cells (c) In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA (d) Since DNA is a hydrophilic molecule it cannot pass through cell membranes

During insetional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the emzyme

The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop product of which of the following enzyme such that colonies do not produce any colour.

The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop product of which of the following enzyme such that colonies do not produce any colour.

After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as (i) Recombinants if colonies are blue-coloured (ii) Recombinants if colonies are blue coloured (iii) Non-recombinants if colonies are blue-coloured (iv) Non - recombinants if colonies are white coloured