Refer to the given steps of DNA strands
(i) Exposure of DNA strands
(ii) Synthesis of RNA primer
(iii) Activation of deoxyribonucleotides
(iv) Chain formatiion
(v) Base pairing
(vi) Proof reading and DNA repair ltviigt DNA polynerase attaches at Ori site
Select the correct sequence of DNA replication.

The replication process involves the following steps: (I) The enzyme DNA-dependent DNA poly merases catalyse the polymerisation of DNA strand. (II) The lagging strand is synthesized in frag ments known as Okazaki fragments, which are later joined by the enzyme DNA Ligase. (III) The origin of replication serves as the recognition site for DNA polymerase and also it provides the site for attachment of RNA primer. (IV)The structural gene coding for a partic ular protein is flanked by the promoter and terminator. Choose the correct option:

Sequences of base pairs in DNA that reads the same on both the strands when the orientation of reading is kept the same are called sequences.

Sequence of base pairs in DNA that reads the same on both the strands when the Orientation of reading is kept the same are called- sequences.

Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

Given below are some step for slicing foreign DNA into a plasmid and then inserting the plasmid into a bacterium. (i) Transform bacteria with recombinant (ii) Cut the plasmid DNA using restriction enzyme . (iii) Extract plasmid DNA to - DNA fragments DNA (iv) Bind the plasmid DNA to non-plasmid DNA fragments . (v) Use ligase to seal plasmid DNA to non- plasmid DNA Arrange the above steps in the right sequence and select the CORRECT option

Differentiate between the following : (i) Leading strand and lagging strand (ii) Prokaryotic DNA and eukaryotic DNA (iii) DNA and RNA (iv) Replication and transcription

Arrange the various steps of DNA fingerprinting technique in the correct order. (i) Separation of DNA fragments by electrophoresis. (ii) Digestion of DNA by restriction endonucleases. (iii) Hybridisation using labelled VNTR prode. (iv) Isolation of DNA . Detection of hybridised DNA fragments by auto-radiography. (vi) Transferring the separated DNA fragments to nitrocellulose membrane.

Arrange the various steps of DNA fingerprinting technique in the correct order. (i) Separation of DNA fragments by electrophoresis. (ii) Digestion of DNA by restriction endonucleases. (iii) Hybridisation using labelled VNTR prode. (iv) Isolation of DNA . Detection of hybridised DNA fragments by auto-radiography. (vi) Transferring the separated DNA fragments to nitrocellulose membrane.

Given below are the steps of DNA fingerprinting in random sequence:- (i) Isolation of DNA. (ii) Separation of DNA fragements by electrophoresis, (iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon. (v) digestion of DNA by restriction endonucleases, (vi). Detection of hybridised DNA fragments by auto radio-graphy. which of the following is the correct sequence:-