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What do you understand by cloning?...

What do you understand by cloning?

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The process of producing genetically similar molecules, cells or organisms from a common processor is called as cloning.
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PRADEEP-BIOTECHNOLOGY: PRINCIPLE'S AND PROCESSES-SHORT ANSWERS QUESTIONS
  1. Explain the role of enzymes nucleases and ligases.

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  2. Expand PCR.

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  3. What do you understand by cloning?

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  4. Expand the following and mention one application of each (i) PCR (ii...

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  5. (a) Mention the difference in the mode of action of exonuclease and en...

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  6. Name the source of the DNA polymerase used in PCR technique. Mention w...

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  7. Write any four ways used to introduce a desired DNA segment into a bac...

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  8. Write the role of 'Ori' and 'restriction' site in a cloning vector pB...

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  9. Explain with the help of a suitable example, the naming of a restricti...

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  10. a) While cloning vectors, which of the two will be preferred by biotec...

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  11. What essential features must be present in a cloning vehicle?

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  12. What are the principal of biotechnology?

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  13. Give a brief historical background of genetic engineering.

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  14. Write a brief account of the enzymes involved in recombinant DNA techn...

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  15. Write short notes on a) Micro injection b) Isolation of genetic materi...

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  16. What is the principle of PCR?

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  17. How is the bacterium Thermus aquaticus employed in recombinant DNA tec...

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  18. (a) What are ‘molecular scissors’ ? Give one. example. (b) Explain t...

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  19. Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

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  20. Explain the importance of a) ori, b) amp^(R ) and c) rop in the E, col...

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