Boliver and Rodriguez developed

Consider the following statements : i. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro ii. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid. iii. Restriction enzymes belong to a class of enzymes called nucleases . Choose that correct option regarding above statements

Which artificial cloning vector was constructed by Boliver and Rodriguez in 1977 ?

F.Boliver and R. Rodrigeuz constructed first artificial vector plasmid was

F.Boliver and R. Rodrigeuz constructed first artificial vector plasmid was

What is development ? What are direct and indirect developments ?

Differentiate between: Direct development and Indirect development.