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How can DNA segments, separated by gel e...

How can DNA segments, separated by gel electrophoresis, be visualized and isolated?

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DNA segments, that have been separated on an agarose gel by electrophoresis are transferred and permanently affixed to a nylon membrane, a process called southern blotting. The DNA bands are invisible to the eye so that they are flagged with some kind of tag that one can visualize. Therefore, a solution containing radioactive probe is spread over the membrane so that they bind to complementary DNA sequences and then can be visualized by autoradiography.
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OSWAAL PUBLICATION-MOLECULAR BASIS OF INHERITANCE -TOPIC-2: Short Answer Type Question- I
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  4. Comment on the utility of variability in number of tandem repeats duri...

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  5. Give an account of mRNA.

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  6. Differentiate between Exons and Introns.

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  7. How is a polypeptide chain synthesis terminated during protein synthes...

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  8. What are structural genes? Name the three structural genes present in ...

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  9. What do you understand by UTR ? Mention its role in translation.

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  10. Enumerate the goals of human genome project.

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  11. Three codons on mRNA are not recognized by transfer RNA. What are they...

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  12. Why is it essential that tRNA binds to both an amino acid and an mRNA ...

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  13. What will these DNA codons be translated into TAC, AAA, ATC? (b) Wh...

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  14. Two petitioner fathers filed case against a lady claiming to be the fa...

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  15. Draw the structure of the initiator tRNA adaptor molecule.

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  16. Why is tRNA called an adaptor molecule?

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  17. One of the codons on mRNA is AUG. Draw the structure of tRNA adaptor m...

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  18. Explain the dual function of AUG codon. Give the sequence of bases it ...

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  19. How can DNA segments, separated by gel electrophoresis, be visualized ...

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  20. What is satellite DNA in a genome ? Explain their role in DNA fingerpr...

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