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Resbiction enzymes present in the clonin...

Resbiction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.

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If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatment with the restriction enzyme.
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OSWAAL PUBLICATION-BIOTECHNOLOGY PRINCIPLES AND PROCESSES -TOPIC -2 PROCESS OF RECOMBINANT DNA TECHNOLOGY (MULTIPLE CHOICE QUESTIONS )
  1. Resbiction enzymes present in the cloning site of a vector should not ...

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  2. Rising of dough is due to :

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  3. An enzyme catalysing the removal of nucleotides from the ends of DNA i...

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  4. The transfer of genetic material from one bacterium to another through...

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  5. Which of the given statement is correct in the context of observing DN...

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  6. 'Restriction' in Restriction enzyme refers to :

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  7. A recombinant DNA molecule can be produced in the absence of the follo...

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  8. In agarose gel electrophoresis, DNA molecules are separated on the bas...

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  9. The most import.mt feature in a plasmid to be used as a vector is :

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  10. While isolating DNA from bacteria, which of the following enzymes is n...

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  11. Which of the following has popularised the PCR (polymerase chain react...

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  12. An antibiotic resistance gene in a vector usually helps in the selecti...

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  13. Significance of 'heat shock' method in bacterial transformation is to ...

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  14. The role of DNA ligase in the construction of a recombinant DNA molecu...

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  15. Which of the following bacteria is not a source of restriction endonuc...

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  16. Which of the following steps are catalysed by Taq polymerase in a PCR ...

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  17. A bacterial cell was transformed with a recombinant DNA that was gener...

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  18. Which of the following should be chosen for best yield if one were to ...

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  19. Who among the following was awarded the Nobel Prize for the developmen...

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  20. Which of the following statements does not hold true for restriction e...

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