Home
Class 12
BIOLOGY
You have created a recombinant DNA molec...

You have created a recombinant DNA molecule by ligating a gene to aplasmid vector. By mistake, your friend adds exonudease enzyme to the tucontaining the recombinant DNA. How will your experimentgel affected as you plan to go for transformation now?

Text Solution

Verified by Experts

The experiment will not likely to be affected as recombinant DNA moleculeis circular closed, with no free ends. Hence, it wiU not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.
Doubtnut Promotions Banner Mobile Dark
|

Topper's Solved these Questions

  • BIOTECHNOLOGY PRINCIPLES AND PROCESSES

    OSWAAL PUBLICATION|Exercise TOPIC -2 PROCESS OF RECOMBINANT DNA TECHNOLOGY (SHORT ANSWER TYPE QUESTIONS-II)|4 Videos
  • BIOTECHNOLOGY PRINCIPLES AND PROCESSES

    OSWAAL PUBLICATION|Exercise TOPIC -2 PROCESS OF RECOMBINANT DNA TECHNOLOGY (LONG ANSWER TYPE QUESTIONS )|5 Videos
  • BIOTECHNOLOGY PRINCIPLES AND PROCESSES

    OSWAAL PUBLICATION|Exercise TOPIC -2 PROCESS OF RECOMBINANT DNA TECHNOLOGY (VERY SHORT ANSWER TYPE QUESTIONS )|11 Videos
  • BIOTECHNOLOGY AND ITS APPLICATIONS

    OSWAAL PUBLICATION|Exercise TOPIC -2 ( Multiple Choice Questions )|14 Videos
  • ECOSYSTEM

    OSWAAL PUBLICATION|Exercise TOPIC-3 : Ecological Succession and Nutrient Cycling (Multiple Choice Questions)|20 Videos

Similar Questions

Explore conceptually related problems

Would you like lo choose an exonuclease enzyme while producing a recombinant DNA molecule?

You have chosen a plasmid as vector for cloning your gene. How ever this vector plasmid lacks a selectable marker. How would it affect your experiment?