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You have created a recombinant DNA molec...

You have created a recombinant DNA molecule by ligating a gene to aplasmid vector. By mistake, your friend adds exonudease enzyme to the tucontaining the recombinant DNA. How will your experimentgel affected as you plan to go for transformation now?

Text Solution

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The experiment will not likely to be affected as recombinant DNA moleculeis circular closed, with no free ends. Hence, it wiU not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.
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