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A foreign DNA and plasmid cut by the sam...

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

A

Polymerase-III

B

Ligase

C

ECO RI

D

Taq polymerase

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The correct Answer is:
B

When cut by the same restriction enyzme, the resultant DNA fragments have the same kind of 'sticky-ends' and these can be joined together (end-to-end) using DNA ligases.
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A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant DNA by :

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NEET PREVIOUS YEAR (YEARWISE + CHAPTERWISE)-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-MCQs
  1. Which of the following restriction enzymes produces blunt ends

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  2. Which of the following is not a component of downstream processing

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  3. A foreign DNA and plasmid cut by the same restriction endonuclease can...

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  4. Stirred-tank bioreactors have been designed for

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  5. Which of the following is a resctriction endonucleus?

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  6. The taq polymerase enzyme is obtained from

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  7. Which of the following is not a feature of the plasmids ?

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  8. The DNA molecule to which the gene of interst is integrated for clonin...

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  9. The cutting of DNA at specific locations became possible with the disc...

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  10. Which vector can clone only a small fragment of DNA ?

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  11. DNA fragments generated by restriction endonucleases in a chemical rea...

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  12. The colonies of recombinant bacteria appear white in contrast to blue ...

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  13. Which one of the following represents a palindromic sequence in DNA?

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  14. The figures below shows three steps (A,B,C) of polymerase chain reacti...

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  15. Biolistics (gene-gun) is suitable for

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  16. For transformation, micro-particles coated with DNA to be bombarded fr...

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  17. PCR and restriction Fragements length Polymorphism are the methods for

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  18. The figure below is the diagrammatic representation of the E.coli vect...

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  19. which one of the following is a case of wrong matching?

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  20. There is a restriction endonuclease called EcoRI. What does 'co' part ...

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