The figure below is the diagrammatic representation of the E.coli vector pBr 322. which one of the given options correctly identifies its certain component (s)

Options

A. `amp^(R),tet^(R)`-antibiotic resistance genes
B. ori-original restriction enzyme
C. rop-reduced osmotic pressure
D. Hindi III, Eco RI-selectable markers

Answer

A

Explanation

E. colic coloning vector pBR332 contains restriction sites (Hindi III, EcoR I, BamH I, Sal I, Pvu II,Pst I, Cla I), ori and antibiotic resistance genes (`amp^(R)` and `tet^(R)`). Rop codes for the restrictions involved in the replication of the plasmid.