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E.coli cloning vector pBR 322 contains r...

E.coli cloning vector pBR 322 contains restriction sites (Hind III,Eco RI, Bam HI, SaI I, Pvu II, Pst I, Cla I), ori, `amp^(R ), tet^(R )`, and rop. In this rop codes for the

A

antibiotic resistance genes

B

foreign DNA

C

selection of recombinants from nonrecombinants

D

proteins involved in the replication of the plasmid

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The correct Answer is:
D
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Match each term on the right with the definition on the left, Column - I A) Bacterial enzymes used to cut DNA at defined sequences B) Sequences cut by restriction enzymes C) Ends left on DNA segments cut by DNA restriction enzymes D) Circular pieces of DNA found in bacteria E) Bacterial viruses F) Process by which bacteria take up pieces of DNA from the environment G) Plasmid DNA that has incorporated human DNA H) Term used to describe any vehicle that moves DNA from the organism to another Column - II I) Transformation II) Cloning vector III) Recombinant IV) Recognition sequences V) Plasmids VI) Sticky ends VII) Restriction enzymes VIII) Bacteriophages

AAKASH SERIES-PRINCIPLES AND PROCESSES -Exercise - III (Previous AIPMT/NEET Questions)
  1. E.coli cloning vector pBR 322 contains restriction sites (Hind III,Eco...

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  2. DNA fragments are:

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  3. The process of separation and purification of expressed protein before...

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  4. The DNA fragments separated on an agarose gel can be visualised after ...

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  5. Stirred tank bioreactors have been designed for

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  6. A foreign DNA and plasmid cut by the same restriction endonuclease ca...

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  7. Which of the following is not a component of downstream processing?

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  8. Which of the following restriction enzymes produces blunt ends?

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  9. The taq polymerase enzyme is obtained from:

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  10. Which of the following is not required for any of the techniques of DN...

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  11. Which of the following is a restriction endonuclease?

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  12. Golden rice is a genetically modified crop plant where the incorporate...

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  13. An analysis of chromosomal DNA using the Southern hybridization techni...

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  14. In vitro clonal propagation in plants in characterized by:

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  15. Which vector can clone only a small fragment of DNA?

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  16. DNA fragments generated by the restriction endonucleases in a chemica...

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  17. The colonies of recombinant bacteria appear white in contrast to blue ...

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  18. For transformation, micro - particles coated with DNA to be bombarded ...

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  19. There is a restriction endonuclease called EcoR l . What does "co" par...

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  20. Agarose extracted from sea weeds finds use in:

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  21. Which one of the following is used as vector for cloning genes into hi...

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